RBS

Part:BBa_K4345015

Designed by: Michelle Patricia   Group: iGEM22_KU_Leuven   (2022-09-30)


pSynSens2.5000 RBS

This RBS was developed using a library and optimised to work robustly in pSynSense2.5000 designed by De Paepe et al. (2018).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

This part is part of the biosensor circuit shown below.


PSynSense2X De Paepe et al 2018.png

Image obtained from De Paepe et. al. (2018)

References

De Paepe, B., Maertens, J., Vanholme, B., & De Mey, M. (2018, April 24). Modularization and Response Curve Engineering of a Naringenin-Responsive Transcriptional Biosensor. ACS Synthetic Biology, 7(5), 1303–1314. https://doi.org/10.1021/acssynbio.7b00419

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Categories
Parameters
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